Current Issue : July - September Volume : 2016 Issue Number : 3 Articles : 4 Articles
Background: There are still a large variety of microorganisms among aquatic animals which have not been\nexplored for their pharmacological potential. Hence, present study was aimed to isolate and characterize a potent\nlactic acid bacterium from fresh water fish sample Zacco koreanus, and to confirm its pharmacological potential.\nMethods: Isolation of lactic acid bacteria (LAB) from fresh water fish samples was done using serial dilution\nmethod. Biochemical identification and molecular characterization of selected LAB isolate 1I1, based on its potent\nantimicrobial efficacy, was accomplished using API kit and 16S rRNA gene sequencing analysis. Further, 1I1 was\nassessed for Ã?±-glucosidase and tyrosinase inhibitory potential as well as antiviral efficacy against highly pathogenic\nhuman influenza virus H1N1 using MDCK cell line in terms of its pharmacological potential.\nResults: Here, we first time report isolation as well as biochemical and molecular characterization of a lactic acid\nbacterium Lactobacillus sakei 1I1 isolated from the intestine of a fresh water fish Z. koreanus. As a result, L. sakei\n1I1 exhibited potent antimicrobial effect in vitro, and diameter of zones of inhibition of 1I1 against the tested\npathogens was found in the range of 13.32 Ã?± 0.51 to 23.16 Ã?± 0.32 mm. Also L. sakei 1I1 at 100 mg/ml exhibited\nsignificant (p < 0.05) Ã?±ââ?¬â??glucosidase and tyrosinase inhibitory activities by 60.69 and 72.59 %, in terms of\nits anti-diabetic and anti-melanogenic potential, respectively. Moreover, L. sakei 1I1 displayed profound\nanti-cytopathic effect on MDCK cell line when treated with its ethanol extract (100 mg/ml), confirming its\npotent anti-viral efficacy against H1N1 influenza virus.\nConclusions: These findings reinforce the suggestions that L. sakei 1I1 isolated from the intestine of fresh water fish Z.\nkoreanus might be a candidate of choice for using in pharmacological preparations as an effective drug....
This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatinine, serum\nuric acid and serum total protein in normal and Staphylococcus aureus infected mice. Thirty seven milk samples were\ncollected from Al-Kut city from cows suffering acute mastitis. After biochemical tests and API Staph System it was\nrevealed that 19 out of 37 milk samples contained S. aureus. Ethanolic extract of pomegranate peels was prepared and\nthe highest in vitro antibacterial effect was observed by 300mg/mL against S. aureus with 30.00 �± 0.57 mm of the\ndiameter zone. Thirty lactating mice were divided into three groups. Group (1): negative control (Normal). Group (2):\npositive control, mice were infected by intramammary injection of S. aureus at 7, 15, 21 and 30 days and left untreated.\nGroup (3): mice infected with intramammary injection of S. aureus and treated orally with 300 mg/kg BW of ethanolic\nextract of pomegranate peel after 48 h of infection for 15, 21 and 30 days. At the end of experiment, blood samples\nwere taken from anaesthetized mice then the mice were killed for histopathological examination and measured the\nfollowing: serum glucose, creatinine, serum uric acid and serum total proteins. Results showed significant increase (P\n<0.05) in the levels of serum glucose, creatinine, serum uric acid and serum total proteins in positive control mice as\ncompared with negative control while pomegranate peel treated group showed a significant (P <0.05) decrease in the\nlevels of serum glucose, creatinine, serum uric acid and serum total proteins. Histological section in mammary gland\nshowed clear pathological effects of S. aureus as compared with those treated with pomegranate peel extracts....
In an attempt to reach better treatment of skin infections, gel formulations containing Cefotaxime (CTX) were prepared. The gel\nwas formulated using Carbopol 934 (C934),Hydroxypropyl Methylcellulose 4000 (HPMC 4000), Carboxy methylcellulose Sodium\n(Na CMC), Pectin (PEC), Xanthan Gum (XG), or Guar Gum (GG). Thirteen different formulas were prepared and characterized\nphysically in terms of color, syneresis, spreadability, pH, drug content, and rheological properties. Drug-excipients compatibility\nstudies were confirmed by FTIR and then in vitro drug release study was conducted. In vitro and in vivo antibacterial activities of\nCTX were studied against wound pathogens such as, Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Pseudomonas\naeruginosa (P. aeruginosa), using either pure drug or Fucidin cream as control. F13 provides better spreadability compared to\nF1 (XG) or F11 (HPMC). Moreover, the release of the drug from hydrogel F13 containing C934 was slower and sustained for 8 h.\nStability study revealed that, upon storage, there were no significant changes in pH, drug content, and viscosity of the gels. Also, F13\nshowed the larger inhibition zone and highest antibacterial activity among other formulations. Histological analysis demonstrated\nthat after single treatment with F13 gel formulation, a noticeable reduction in microbial bioburden occurred in case of both Gram\npositive and Gram negative bacterial isolates....
Pterospartum tridentatum and Mentha pulegium are largely used in Portuguese folk medicine to treat several human disorders\nand inflammatory processes but without any consistent evidence for those beneficial pointed properties. Thus, the aim of the\ncurrent work is to evaluate its benefits and phytochemicals related to those beneficial properties. A distinct polyphenol profile\nbetween P. tridentatum and M. pulegium was found. Taxifolin, myricetin, ginestin, ginestein, and ginestein derivatives, biochanin\nA-glucoside, and biochanin A were identified in P. tridentatum, whilst in M. pulegium the luteolin-7-rutinoside, diosmin, and\napigenin and respective derivatives were most representative polyphenols. These variations had implications in the antiradical and\nantibacterial activity and the P. tridentatum exhibited the highest antibacterial activity against methicillin-resistant and methicillinsensitive\nStaphylococcus aureus MSSA, which was mainly dose-dependent. This antibacterial activity seems to be related to high\ncontent of flavonols, flavones, and is oflavones, which can act synergistically with each other against this type of bacteria. Our results\nshowed consistent evidence that Pterospartumtridentatumand Mentha pulegium are an important reservoir of phytochemicals with\nantiradical activity and antibacterial capacity and thus they might be used in a preventive way or in a combined pharmaceutical\nand antibiotic therapy against pathogenic bacteria....
Loading....